3′ → 5′ Exonucleases of DNA Polymerases ϵ and δ Correct Base Analog Induced DNA Replication Errors on Opposite DNA Strands in Saccharomyces cerevisiae
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منابع مشابه
3 ' + 5 ' Exonucleases of DNA Polymerases E and 6 Correct Base Analog Induced DNA Replication Errors on Opposite DNA Strands in Saccharomyces
The base analog 6-Nhydroxylaminopurine (HAP) induces bidirectional GC + AT and AT + GC transitions that are enhanced in DNA polymerase E and 6 3' + 5' exonuclease-deficient yeast mutants, po12-4 and pol?-01, respectively. We have constructed a set of isogenic strains to determine whether the DNA polymerases 5 and E contribute equally to proofreading of replication errors provoked by HAP during ...
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Three major DNA polymerases replicate the linear eukaryotic chromosomes. DNA polymerase α-primase (Pol α) and DNA polymerase δ (Pol δ) replicate the lagging-strand and Pol α and DNA polymerase ε (Pol ε) the leading-strand. To identify factors affecting coordination of DNA replication, we have performed genome-wide quantitative fitness analyses of budding yeast cells containing defective polymer...
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متن کاملBoth DNA Polymerases δ and ε Contact Active and Stalled Replication Forks Differently.
Three DNA polymerases, polymerases α, δ, and ε (Pol α, Pol δ, and Pol ε), are responsible for eukaryotic genome duplication. When DNA replication stress is encountered, DNA synthesis stalls until the stress is ameliorated. However, it is not known whether there is a difference in the association of each polymerase with active and stalled replication forks. Here, we show that each DNA polymerase...
متن کاملFunctions of Saccharomyces cerevisiae 14-3-3 proteins in response to DNA damage and to DNA replication stress.
Two members of the 14-3-3 protein family, involved in key biological processes in different eukaryotes, are encoded by the functionally redundant Saccharomyces cerevisiae BMH1 and BMH2 genes. We produced and characterized 12 independent bmh1 mutant alleles, whose presence in the cell as the sole 14-3-3 source causes hypersensitivity to genotoxic agents, indicating that Bmh proteins are required...
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ژورنال
عنوان ژورنال: Genetics
سال: 1996
ISSN: 1943-2631
DOI: 10.1093/genetics/142.3.717